TABIY PROTEINLAR: MANBALARI VA QO'LLANISHI
Keywords:
Aminokislotalar, tabiiy oqsillar, SDS gel elektroforez, tuzlashAbstract
Butun dunyoda o'simlik oqsili oziq-ovqat manbai sifatida katta hissa qo'shadi, chunki u inson fiziologik ehtiyojlarini qondirish uchun muhim aminokislotalarni o'z ichiga oladi. Biroq, ko'p qirrali o'simlik oqsillari biotexnologiyaning molekulyar vositalaridan foydalangan holda ishlab chiqarilganligi sababli dorivor vositalar sifatida ishlatiladi. Proteinlarni o'simliklar, hayvonlar va mikroorganizm hujayralaridan olish mumkin. Ko'p miqdorda iqtisodiy oqsillarni o'simlik urug'idan olish mumkin. Ushbu tabiiy oqsillar oqsillarning fizik-kimyoviy xususiyatlariga qarab izolyatsiyalash usullari bilan olinadi. O'zaro bog'liq bo'lmagan oqsillar aralashmalarini o'z ichiga olgan hujayralardan bitta oqsilni ajratish va tozalash oqsillarning fizik va kimyoviy xususiyatlari tufayli amalga oshiriladi.
References
1. Hermann JR. Protein and the Body. Oklahoma Cooperative Extension Service, Division of Agricultural Sciences and Natural Resources. Oklahoma State University: T–3163–1 – T–3163–4.
2. McArdle WD, Katch FI, Katch VL. Vitamins, minerals, and water, In: Exercise physiology: Energy, Nutrition, and Human Performance, 5th ed. Philadelphia, PA: Lippincott Williams and Wilkins; 2001. p. 47-81.
3. Millward DJ. The nutritional value of plant based diets in relation to human amino acid and protein requirements. Proc Nutr Soc 1999a; 58:249-60.
4. Streitwieser A, Jr. Heathcock CH. “Introduction to organic chemistry” 3rd ed., 1985.
5. Furst P, Stehle P. "What are the essential elements needed for the determination of amino acid requirements in humans?” J Nutr 2004;134 (6 Suppl):1558-65S.
6. Satyanarayana U, Chakrapani U. Books of Biochemistry. 3- ed., 2006.
7. Bachmair A, Finley D, Varshavsky A. In vivo half-life of a protein is a function of its amino-terminal residue. Science 1986;234:179-86.
8. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and predict the molar absorption coefficient of a protein. Protein Sci 1995;11:2411-23.
9. Edelhoch H. Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 1967;6:1948-54.
10. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 1989;182:319-26.
