LABORATORIYADA PCR APPARATI BILAN ISHLASH ASOSIY JARAYONLAR VA AMALIY MASLAHATLAR
Keywords:
PCR, Termotsikler, DNK amplifikatsiyasi, Laboratoriya texnologiyasi, Klinik diagnostika, Reagentlar, Laboratoriya amaliyotlari.Abstract
Ushbu maqola laboratoriyada PCR (Polymerase Chain Reaction) apparati bilan ishlash jarayonlarini, uning asosiy qismlari, bosqichlari va amaliy maslahatlarini yoritadi. PCR laboratoriyada DNKning ma’lum qismlarini tez va aniq ko‘paytirish imkonini beradi, bu esa klinik diagnostika, genetik tadqiqotlar va infeksion kasalliklarni aniqlashda muhim ahamiyatga ega. Maqolada laboratoriya ishini samarali va ishonchli tashkil etish uchun zarur bo‘lgan texnik qoidalar, reagentlar bilan ishlash tartibi va nazorat namunalaridan foydalanish tavsiyalari keltirilgan.
References
1.Lorenz TC. Polimeraza zanjiri reaktsiyasi: asosiy protokol va muammolarni bartaraf etish va optimallashtirish strategiyalari. J Vis Exp. 2012 yil 22-may;(63):e3998. [ PMC bepul maqola ] [ PubMed ]
2.Ramesh R, Munshi A, Panda SK. Polimeraza zanjiri reaktsiyasi. Natl Med J Hindiston. 1992 yil may-iyun; 5 (3): 115-9. [ PubMed ]
3. Dieffenbach CW and Dveksler GS. Setting up a PCR laboratory. In PCR Methods and Applications, Cold Spring Harbor Laboratory Press. Genome Research,1993; 3 : S2-S7 http://genome.cshlp.org/content/3/2/S2.refs.html
4.Newton CR. 1995. Setting up a PCR laboratory. In PCR: Essential data. (ed. C.R. Newton), p. 216. Wiley, New York.
5.Mifflin TE. Setting up a PCR laboratory. www.biosupplynet.com/pdf/01
6.Roux KH. Optimization and trouble shooting in PCR. In PCR Methods and Applications, Cold Spring Harbor Laboratory Press. Genome Research, 1995; 4 :S185-S194 http://genome.cshlp.org/content/4/5/S185.refs.html
7.Kubista M, Andrade JM, Bengtsson M et al. 2006. The Real Time PCR. Mol Aspects Med; 27: 95-125
8. Millar BC, Xu J, Moore JE. 2007. Molecular diagnosis of medically important bacterial infections. Curr Issues Mol Biol;9: 21-39
9. Xaydarali ro‘ziboyev Nafisa Komilova Gulbahor Radjabova “BIOINFORMATIKA” Toshkent-2022 .174-bet